Arabidopsis thaliana, as a small model plant, has become a star in plant biology research. Its small size, short life cycle, and relatively simple genome… Read More »Application of stereo fluorescence microscopy in Arabidopsis research

Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish details in a sample observed under a microscope. NA is a unitless… Read More »Importance of Numerical Aperture in Microscopy

01 Introduction to optical microscopy Optical microscopes are important means and tools for people to observe small objects and understand the microscopic world. They are also… Read More »IApplication of Infinity Corrected Microscope Objectives

Three-dimensional imaging is a commonly used technology in life sciences, chemistry, materials and other research. However, conventional three-dimensional imaging technology requires layer-by-layer scanning of the… Read More »Digital holographic microscope: three-dimensional dynamic tracking and topography measurement

Darkfield microscopy definition Darkfield microscopy is actually a microscope that uses dark-field illumination. It is a special microscope that can distinguish the details of the… Read More »Stars in the Dark Field: Dark Field Scattering and Applications of Metal Nanoparticles

Laser scanning imaging basics Unlike wide-field microscopes, which collect full-field images simultaneously through flood illumination and cameras, laser scanning microscopes usually can only use single-focus… Read More »Laser scanning microscope

In a confocal spectrometer, it is advantageous to combine multiple complementary spectroscopic techniques to maximize the information produced by a single sample. Two widely used… Read More »How to realize Raman imaging/photoluminescence (PL) imaging/phosphorescence lifetime (PLIM) imaging on a Raman spectrometer?

In 1928, Indian scientist C.V. Raman observed a phenomenon: when light passes through a transparent medium, the frequency of part of the scattered light changes.… Read More »The principle, three major characteristics of Raman spectroscopy and the six major differences between Raman spectroscopy and infrared spectroscopy

In organisms, the intercellular substance and body fluid components within cells constitute the microenvironment for cell survival. A stable microenvironment is the most important condition… Read More »What is Fluorescence Lifetime Imaging FLIM?

Overview 1. An upright microscope can be used for most samples, but large-volume samples or samples that need to be placed from above during imaging… Read More »What are optical microscopy and the three most common illumination techniques used in optical microscopy?