I. What is a Polarizing Microscope?
A polarizing microscope is a type of microscope that utilizes polarized light to study the optical properties of materials. It is primarily used in geological research to study geological specimens, hence it’s also referred to as a petrographic microscope. Recently, it has found broader applications in medicine and biology as well.
The characteristic feature of a polarizing microscope is its ability to transform ordinary light into polarized light for microscopic examination, aiming to identify whether the material exhibits single or double refraction. Double refraction is a fundamental characteristic of crystals. Therefore, polarizing microscopes have extensive applications in mineralogy, polymers, fibers, glass, semiconductors, and chemistry.
In biology, many structures also exhibit double refraction, necessitating the use of polarizing microscopes to distinguish them. In botany, this includes the identification of fibers, chromosomes, spindle bodies, starch grains, cell walls, and crystals in cytoplasm and tissues. In plant pathology, the invasion of pathogens often leads to changes in tissue chemical properties, which can be identified using polarizing microscopes.
II. Principles of Polarizing Microscopes:
The principle of a polarizing microscope involves passing light through a polarizer, followed by passing it through the sample. The transmitted light perpendicular to the polarizing plane will be blocked by the analyzer. The light passing through the analyzer typically has linear polarization perpendicular to the direction of the analyzer. When observing an object through a polarizing microscope, you can see two images of the same object. One image will appear dark, and the other bright. This is because light waves become polarized as they reflect off different surfaces and angles, so they do not all have the same orientation when they reach your eyes.
III. Reflective and Transmissive Polarizing Microscopes:
Polarizing microscopes are used to observe chemicals, rocks, and minerals through transmitted and/or reflected light. Reflective polarizing microscopes are used to examine opaque samples, also known as ore microscopes; transmissive polarizing microscopes are used to examine transparent or semi-transparent samples, also known as petrographic microscopes. Apart from transmissive polarizing microscopes, reflective and transmissive polarizing microscopes are the most commonly used types in biological, cytological, histological, pharmaceutical, and pharmacological research.
IV. Performance of XPL-2 Series Polarizing Microscopes:
- Conventional detection using transmissive/reflected polarizing microscopes.
- Widely applied in crystallography, geology, analytical chemistry, medicine, biosciences, forensics, environmental sciences, pharmaceuticals, toxicology, asbestos analysis, and more.
- Capable of single polarization, orthogonal polarization, and colonoscopic observation.
V. Technical Specifications of XPL-2 Series Polarizing Microscopes
XPL-2 Technical Specifications
Observation system | Flat pull binocular observation head with an inclination angle of 45 ° |
Flat pull three eye observation head with an inclination angle of 45 ° | |
eyepiece | WF10X/18 |
WF10X/18 Cross Eyepiece | |
Reticle board | |
Grid board | |
objective lens | Stress free achromatic objective lenses 4X/0.10/-, 10X/0.25/- |
Stress free achromatic objective lenses 25X/0.40/0.17, 40X/0.65/0.17, 63X/0.85/0.17 | |
Stress free flat field achromatic objective 25X/0.40/0, 40X/0.65/0, 63X/0.85/0 | |
Lamp room calibration objective lens | |
Converter | Internal four hole converter with adjustable center |
Deviation detection device | 0 ° -90 ° rotary polarizer |
Bulbous mirror | Bulbous mirror, adjustable center, adjustable focus, and movable optical path |
Optical path compensator | λ Test board (first level red), 1/4 λ Test board, quartz wedge test board |
Circular rotating loading platform | diameter Φ 160mm, scale value 1 °, vernier value 6 ‘ |
Spotlight mirror | NA1.25 Abbe type condenser with variable light bar and filter |
Focus adjustment | Coaxial coarse and micro focusing mechanism, focusing range of 26mm, fine adjustment grid value of 0.002mm |
Deviation device | Polarizing mirror, capable of 360 ° rotation |
lighting system | 12V/20W halogen lamp with adjustable brightness (for transmission) |
12V/50W halogen lamp with adjustable brightness (for reflection) | |
attachment | Mechanical moving ruler, movement range: 30X40mm (including slicing test piece holder) |
Flattening machine | |
Micrometer ruler |
XP-221 Technical Specifications
Observation system | Flat pull binocular observation head with an inclination angle of 45 ° |
Flat pull three eye observation head with an inclination angle of 45 ° | |
eyepiece | WF10X/18 |
WF10X/18 Cross Eyepiece | |
Reticle board | |
Grid board | |
objective lens | Stress free achromatic objective lenses 4X/0.10/-, 10X/0.25/- |
Stress free achromatic objective lenses 25X/0.40/0.17, 40X/0.65/0.17, 63X/0.85/0.17 | |
Stress free flat field achromatic objective 25X/0.40/0, 40X/0.65/0, 63X/0.85/0 | |
Lamp room calibration objective lens | |
converter | Internal four hole converter with adjustable center |
Deviation detection device | 0 ° -90 ° rotary polarizer |
Bulbous mirror | Bulbous mirror, adjustable center, adjustable focus, and movable optical path |
Optical path compensator | λ Test board (first level red), 1/4 λ Test board, quartz wedge test board |
Circular rotating loading platform | diameter Φ 160mm, scale value 1 °, vernier value 6 ‘ |
Focus adjustment | Coaxial coarse and micro focusing mechanism, focusing range of 26mm, fine adjustment grid value of 0.002mm |
Deviation device | Polarizing mirror |
lighting system | 12V/50W halogen lamp with adjustable brightness (for reflection) |
attachment | Mechanical moving ruler, movement range: 30X40mm (including slicing test piece holder) |
Flattening machine | |
Micrometer ruler |